Life Science Technician 2

School of Humanities and Sciences, Stanford, California, United States

Research

Post Date May 17, 2024

Requisition # 100640

Life Science Technician 2

The School of Humanities and Sciences (H&S) is the foundation of a liberal arts education at Stanford. The school encompasses 23 departments and 25 interdisciplinary programs. H&S is home to fundamental and applied research, where free, open, and critical inquiry is pursued across disciplines. As the university’s largest school, H&S serves as the foundation of a Stanford undergraduate education no matter which discipline students pursue as a major. Graduate students work alongside world-renowned faculty to pursue and shape foundational research that leads to breakthroughs and discoveries that shed new light on the past, influence the present, and shape the future. Together, faculty and students in H&S engage in inspirational teaching, learning, and research every day.

Department/Program Description: The Dixon lab (http://www.dixonlaboratory.com) has a position for a motivated research assistant at the Life Science Technician 2 level with interests in cancer, cell biology, or chemical biology. Our lab focuses on identifying and characterizing new cell death pathways (e.g. ferroptosis) that can potentially be activated to treat cancer. A major goal of the lab is to understand how new small molecules we have discovered in the lab can induce ferroptosis in cancer cells and seeking to understand whether this involves known or novel molecular mechanisms. Research in the lab is driven by in vitro approaches with a particular focus on the effects of different small molecules on cancer cell death.

Position Summary: This position will provide opportunities to learn a range of cell biology and chemical biology techniques, and offers a unique opportunity to collaborate with other scientists interested in genomics, biochemistry and computational biology. This is a 1 year fixed term position with the possibility of renewal.

The main responsibilities of the successful candidate will be culturing cancer cells of different genotypes and examining the effects of lethal compound treatment. In addition, follow-up studies examining the effects of deleting specific genes using CRISPR/Cas9 technology on drug-induced cell death will be pursued. Other responsibilities will include performing routine laboratory techniques such as DNA cloning, as well as maintaining excellent records of lab work, curating samples, and assisting in data analysis. This is a two-year fixed-term appointment with the possibility to renew.

This is a 100% FTE, 1 year fixed term, bargaining unit position. This position will be based on the Stanford campus.

If you believe that this opportunity is a match for your knowledge, skills and abilities, we encourage you to apply. Thank you for considering employment opportunities with the School of Humanities and Sciences.

Core Duties:

● Perform animal surgical techniques such as suturing, catheterization and infusion of arteries, small organ dissection, organ perfusion and tumor implantation and passage in small animals.

● Immunohistological procedures including frozen sectioning and multi-step immunologic staining for fluorescence or enzymatic detection. Some level of histologic assessment may be required.

● Maintenance, transfer and establishment of cell lines from normal or tumor cells, preparation and bioassay of serum, screening for contamination, isolation of cell mutants, cell staining, mass production of cells and cell cloning.

● Fixation, dehydration, staining and embedding of specimens for electron microscopy; carbon coating, negative staining, shadowing, operation of electron microscope (EM) either transmission or scanning; use of an ultramicrotome with a diamond knife for routine thin sectioning; alignment of an EM where the system is automated.

● Purification and /or structural characterization of compounds for which a protocolis well established.

● Separation of cells, organelles, or molecules using density gradients.

● Radioimmunoassay of viruses, proteins, or hormone levels in cell extract according to established protocol.

● Techniques such as electrophoresis, chromatography, autoradiography, radiolabeling of animals, cell cultures, or macromolecules.

● Isolation, purification and maintenance of mammalian viruses and bacteriophages; determination of viral concentration.

● Genetic manipulation of bacteria including mutagenesis, ‘phage transduction and transformation.

● Accurate documentation of experimental protocols and basic statistical analysis of data.

• Other duties may also be assigned.

This position is represented by SEIU Local 2007 and the collective bargaining agreement between the university and SEIU Local 2007 governs the terms and conditions of employment.

Education and Experience:

Two years of laboratory experience or two years of college coursework in the scientific discipline a combination of equivalent education and relevant work experience.

Minimum Qualifications:

Work at the Life Science Technician II level requires experience and ability beyond that required for the Life Science Technician I, including increased technical skills and problem-

solving abilities.

Physical Requirements:

● Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand.

● Ability to stand and move on hard surfaces for up to eight hours.

● Occasionally lift and handle materials up to 10 pounds.

● Rarely carry/pull and/or push up to 20 pounds.

● Must have correctible vision to perform duties of the job.

● Position may require repetitive motion.

Working Conditions:

Work occurs in a research laboratory environment. Depending on research, may work in the vicinity of animals, chemicals, gasses, or hazardous materials. Expected to follow all safety requirements and procedures including wearing appropriate Personal Protective Equipment (PPE) if required.

Work Standards:

· Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.

· Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors based on training and lessons learned.

· Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University’s Administrative Guide,

http://adminguide.stanford.edu/. and the appropriate collective bargaining agreement at https://cardinalatwork.stanford.edu/hr-processes-policiespolicies/labor-relations-collective-bargaining.

The expected pay range for this position is $35.41 (Step 1) to $ 42.63 (Step 10) per hour. Placement in the pay range is subject to the applicable collective bargaining agreement.

Stanford University provides pay ranges representing its good faith estimate of what the university reasonably expects to pay for a position.

Why Stanford is for You

Imagine a world without search engines or social platforms. Consider lives saved through first-ever organ transplants and research to cure illnesses. Stanford University has revolutionized the way we live and enrich the world. Supporting this mission is our diverse and dedicated 17,000 staff. We seek talent driven to impact the future of our legacy. Our culture and unique perks empower you with:

● Freedom to grow. We offer career development programs, tuition reimbursement, or audit a course. Join a film screening, or listen to a renowned author or global leader speak.

● A caring culture. We provide superb retirement plans, generous time-off, and family care resources.

● A healthier you. Climb our rock wall, or choose from hundreds of health or fitness classes at our world-class exercise facilities. We also provide excellent health care benefits.

● Discovery and fun. Stroll through historic sculptures, trails, and museums.

● Enviable resources. Enjoy free commuter programs, ridesharing incentives, discounts and more.

The job duties listed are typical examples of work performed by positions in this job classifications and are not designed to contain or be interpreted as a comprehensive inventory of all duties, tasks and responsibilities. Specific duties and responsibilities may vary depending on department or program needs without changing the general nature and scope of the job or level of responsibility. Employees may also perform other duties as assigned.

Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of their job.

Stanford is an equal employment opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law.

Additional Information

• Schedule: Full-time

• Job Code: 5644

• Employee Status: Fixed-Term

• Grade: A24

• Requisition ID: 100640

• Work Arrangement : On Site