Description

The Environmental Cardiology and Vascular Biology Laboratory is seeking to hire a Research Assistant to perform experiments in the areas of vascular and molecular biology, lipidology, pathology, and toxicology. Experiments include: histological analyses of aortic and liver sections for atherosclerotic lesions and hepatic steatosis; pulmonary macrophage transplantation studies; bone marrow transplantation studies; assessment of gene expression by quantitative PCR in lungs, liver and aortas of animals exposed to air pollutants and e-cigarettes. Primary duties:

*Conduct molecular biological procedures such as DNA and RNA extraction, gel electrophoresis, genotyping, western blots, real-time PCR, ELISAs, FPLC, ultracentrifugation

*Determine tissue and cellular oxidative stress through various techniques, including assessment of oxidized lipids by mass spectrometry. Perform tissue culture experiments and analyze histological samples

*Mouse colony management

*Perform bleeding, bronchoalveolar lavage, harvest of peritoneal macrophages, culture of bone marrow-derived macrophages, intratracheal administration of compounds, tissue and organ isolation

*Assist with preparation of manuscripts, grant submissions, and figures

Salary range: $28.04-$45.11 HourlyQualifications

*Knowledge and proficiency in cellular and molecular biology techniques

*Ability to perform tissue culture experiments

*Ability to perform animal dissections

*Experience with mouse colony management

*Ability to perform technical procedures in a timely and accurate manner

*Knowledge and ability to operate basic laboratory equipment such as balances, pH meter, pipettes, light microscopes, centrifuges

*Ability to operate laboratory equipment such as FPLC/HPLC, ultracentrifuge

*Ability to follow written protocols and maintain records and logs accurately.

*Proficient in Microsoft Office Suite

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