Job ID 12454 Location Case Main Campus Full/Part Time Full-Time Regular/Temporary Regular Job Description POSITION OBJECTIVE Working under moderate supervision, the research assistant 1 will perform research work toward understanding the role of the GOLPH3 pathway in Golgi secretory trafficking and intracellular signaling. The research assistant will perform trafficking and signaling assays in cell culture. The research assistant will also be involved in managing a transgenic mouse colony. This position will work with animals. ESSENTIAL FUNCTIONS Perform research work involving use of laboratory skills by following research plan and procedures and protocols. Use model cell culture systems, immunoprecipitation, Western blotting, and mass spectroscopy to identify and quantify signaling protein complexes, and measure signal transduction pathway activation under defined conditions. (10%) Conduct analysis of samples using established methods and techniques, including generating, storing, and analyzing samples, and quantifying and presenting the resulting data in pictoral or graphical format. Techniques used include sterile cell culture and expression of recombinant proteins by transfection with specific plasmid DNAs; siRNA transfection for knockdown of specific proteins, SDS-PAGE, immunoblotting, and quantification of specific proteins from immunoblots; immunofluorescence, confocal microscopy; and basic statistics. (40%) Under guidance, assist in managing a transgenic mouse colony, including setting up breeding, weaning of litter, and recording of data using database software. (10%) Record results for each individual experimental attempt in personal notebook (paper and electronic), including detailed procedures used, results obtained, and analysis performed. (10%) Maintain records of all experiments on the lab data server. (10%) Maintain test equipment utilized in experiments and maintain work area in an organized and clean state. (10%) NONESSENTIAL FUNCITONS Order laboratory supplies as needed (4%) Maintain up-to-date laboratory safety training (1%) Maintain familiarity with other work ongoing in the lab by reading research papers and attending weekly lab meetings. (3%) Perform other duties as assigned. (2%) CONTACTS Department: Daily contact with supervisor to discuss research. Frequent (daily/weekly) contact with other lab members to discuss overall research within the group, and specific research, problems, and solutions. University: Occasional contact with other departments to share samples and information. External: Occasional contact with external collaborators to share samples and information. Students: Occasional contact with student employees and volunteers. SUPERVISORY RESPONSIBILITIES This position has no direct supervision of staff employees. QUALIFICAITONS Education/Experience: Bachelor's degree in science and 0 to 1 year of experience or Associate's degree in an approved biotechnology program and 0 to 2 years of experience required. REQUIRED SKILLS Has knowledge of commonly used concepts, practices, and procedures within the field of post-translational modifications and cellular signaling. Relies on instructions and pre-established guidelines to perform the functions of the job. Ability to operate laboratory equipment, including autoclave, deep freezer, centrifuges, spectrophotometer, and electrophoresis. Must demonstrate compliance with university animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AALAC guidelines and other applicable regulatory guidelines. Must demonstrate compassion for animals within university facilities and dedication to the Animal Resource Center's mission. Must handle animals with care and respect at all